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. 2018 Aug 22;46(20):10930–10945. doi: 10.1093/nar/gky758

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Generation of mtu1 knock-out zebrafish using CRISPR/Cas9 system. (A) Schematic representation of CRISPR/Cas9 target site at exon 4 as used in this study. An allele, mtu1^ins32bp was produced by a 32 bp insertion in exon 4 and a truncated 145 amino acid non-functional protein. (B–D) Genotyping of mtu1^ins32bp by Sanger sequence, the PAGE RFLP and Western blot analyses. (E) The morphology of mtu1^−/−, mtu1^+/− and mtu1^+/+ zebrafish at 3 dpf. (F) The ratios of genotypes/phenotype of offsprings (F2) in clutches from different F1 mtu1 heterozygous crosses at 10 dpf (n = 350).