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. 2018 Aug 22;46(20):10930–10945. doi: 10.1093/nar/gky758

Figure 6.

Figure 6.

Enzymatic activities of respiratory chain complexes and measurement of ATP levels. (A) The activities of respiratory complexes were investigated by enzymatic assays on complexes I, II, III, IV and V in mitochondria isolated from mtu1 mutant and wild type zebrafish. The calculations were based on three independent determinations. (B) Measurement of mitochondrial and cytosolic ATP levels. Average cytosolic ATP level (presence of oligomycin for inhibition of the mitochondrial ATP synthesis) and mitochondrial ATP level (subtraction of cytosolic ATP level from total cellular ATP levels) are shown. Three independent experiments were made for each genotype of zebrafish. Graph details and symbols are explained in the legend to Figure 3.