Figure 8.
Reduced density of hair cell bundles in inner ear labyrinth. (A) Anatomy of adult wild type zebrafish inner ear labyrinth, showing two lagenas (L), two saccule (S), and two utricle (U). (B–D) Hair bundle density in lagenas, saccule and utricle. The hair cells, nuclei of from mutant and wild type zebrafish were stained with phalloidin (green) and DAPI (blue), respectively. Hair cell counts were sampled at six locations of lagenas, four locations of saccule and six locations of utricle, respectively. A 1600 μm2 box was placed at each sampling area and labeled hair cell bundles were counted within each box to determine hair cell density were selected for counting. A, anterior; D, dorsal; M, medial; P, posterior; V, ventral. (E). Quantification of density of hair cell bundles for the mtu1−/−, mtu1+/− and mtu1+/+ zebrafish. Graph details and symbols are explained in the legend to Figure 3.