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. 2018 Aug 22;46(20):10930–10945. doi: 10.1093/nar/gky758

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 9. — Mitochondrial defects in hair cells. (A) Assessment of mitochondrial function in hair cells by enzyme histochemistry (EHC) staining for SDH and COX in the frozen-sections of posterior macula of mtu1^−/−, mtu1^+/− and mtu1^+/+ zebrafish at 5dpf. Loss of EHC signal is indicated by arrows (magnification X400). V, ventral; L, lateral; Ov, otic vesicle; Hc, hair cell; Sc, supporting cell. (B) Mitochondrial networks from hair cells of inner electron microscopy. Ultrathin sections were visualized with 5000×, 10 000×, 20 000× and 60 000× magnifications. (C) Quantification of mitochondrial numbers of hair cells from the mtu1^−/−, mtu1^+/− mutant and mtu1^+/+ zebrafish. The calculations were based on 50 different hair cells of mtu1^−/−, mtu1^+/− mutant and mtu1^+/+ zebrafish, respectively. Graph details and symbols are explained in the legend to Figure 3.