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. 2018 Aug 30;46(20):11002–11013. doi: 10.1093/nar/gky779

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Subcellular distribution of AATF, TAP26, NOL10, IFI16, NGDN and KU70 in control and ActD or CX5461-treated cells. Immuno-localisation of AATF (A), FLAG-tagged TAP26 (B), NOL10 (C), IFI16 (D), FLAG-tagged NGDN (E) and KU70 (F) in U-2 OS cells. Fibrillarin and Hoechst staining were used as nucleolar and nuclear markers respectively. The subcellular distribution of each protein was monitored in control cells and in cells treated with actinomycin D (ActD) or CX5461. Each of the candidate RNAPI-dependent RBPs is present in the nucleolus. The size bar represents 10 microns.