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. 2018 Sep 13;46(20):10757–10770. doi: 10.1093/nar/gky829

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — gfp-fused rpa2 allele construction and functional characterization. (A) Representation of the chromosomal locus of the gfp⁺::rpa2⁺ allele. The regions of homology between the plasmid and the chromosome used for pop-in/pop-out gene replacement are represented by white boxes [Up-Stream (US) and Down-Stream (DS) regions]. Surviving fractions for rpa2⁺ (▪) and gfp⁺::rpa2⁺ cells (•) in response to (B) increasing doses of UV irradiation, (C) increasing concentrations of phleomycin and (D) increasing concentrations of aphidicolin. Error bars represent standard deviations of at least three independent experiments.