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. 2018 Sep 13;46(20):10757–10770. doi: 10.1093/nar/gky829

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — In vivo localization of Hoechst-labeled DNA and GFP-labeled RPA2 using wide-field microscopy. A total of 274 control cells, 159 cells exposed to 5 μg/ml aphidicolin, and 389 cells exposed to 100 μg/ml phleomycin, were analyzed. (A) Images for each condition of the Hoechst-labeled DNA, the GFP signal and the merge image of the DNA signal in cyan and the GFP signal in magenta. Bar equals 5 μm. (B) Mean intensity (A.U.) and (C) Maximum intensity (A.U.) of the Hoechst-labeled DNA signal in cells depending on the individual number of GFP::RPA2 foci. Light histograms correspond to control cells, grey ones to aphidicolin-treated cells and orange ones to phleomycin-treated cells. Error bars represent standard deviations of at least three independent experiments. Unpaired t-test with Welch's correction were performed in comparison to control cells. ****Significantly different, P < 0.0001; **Significantly different, P < 0.01.