Fig. 6.

MUC20 knockdown inhibits HGF/MET signalling in PDAC cells. a MUC20 knockdown decreased phospho-MET levels. MUC20 was knocked down with siRNA (si-MUC20-1) in HPAF-II cells cultured with PSC-conditioned medium. Changes in 49 phospho-RTKs were analysed by western blotting of human phospho-RTK array (R&D Systems). b MUC20 knockdown inhibited phosphorylation of MET and AKT in HPAC and HPAF-II cells treated with 25 ng/ml HGF. c MUC20 knockdown inhibited HGF-triggered migration and invasion in HPAC and HPAF-II cells analysed by Transwell migration assay and Matrigel invasion assay, respectively. Scale bars indicate 1 mm. d MUC20 knockdown significantly reversed HGF-enhanced viability in HPAC and HPAF-II cells. Cells cultured in 10% FBS/DMEM-F12 were treated with PBS, MET inhibitor PHA665752 (2.5 µM) or HGF (25 ng/ml), as indicated. Viability was analysed by the MTT assay. Data are presented as mean (n = 3) ± SD. *P < 0.05; **P < 0.01