Fig. 5.

RanBP2 regulates the localization and antiviral restriction activity of human endogenous TRIM5α. a RanBP2 regulates the subcellular localization of TRIM5α in human myotubes. The localization of RanBP2 (top), and TRIM5α (bottom) were assessed in myoblasts and myotubes using confocal imaging. Scale bar = 20 μm. b RanBP2 regulates the subcellular localization of TRIM5α in human dendritic cells. Human monocyte-derived DCs were transduced with a RanBP2 shRNA (shBP2) or empty vector (shE). Knock-down was assessed at d7 by qRT-PCR and DCs were labelled with an α-TRIM5α antibody. Three representative fields are shown for each condition. Scale bar = 5 μm. c RanBP2 regulates the antiviral activity of endogenous TRIM5α. HeLa cells, which naturally restrict N-MLV, were lipofected with an siRNA against RanBP2 or control (siBP2 and siCtrl, respectively) and after 2 days were infected with increasing doses of N-MLV-YFP (MOI 1, 5, 10). Images were acquired at 2 dpt by widefield microscopy. The graph shows the mean fluorescence intensity (MFI) in representative fields at MOI 1. Scale bar = 100 μm