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Actinobacteria lipoglycans bearing (α1 → 2)-linked dimannoside caps bind and induce signaling via Dectin-2. Lipoglycans (1 µg in (A) from 300 to 10 ng in (B) 0.1 µg in (C)) were tested for their capacity to bind Dectin-2-Fc (A), and to induce NF-κB activation in HEK-Dectin-2 cells (B) and TNF-α production by BMDCs (C). Conditions are the same as in Fig. 2. Data show mean ± SEM. NC, non-coated.
