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. 2018 Nov 15;9(12):1139. doi: 10.1038/s41419-018-1183-8

Fig. 5. lncRNA AC003092.1 functions as ceRNA and negatively modulates miR-195 expression.

Fig. 5

a Cellular localization of lncRNA AC003092.1 in U87, U87TR, U87TR-V-AC, U251, U251TR, and U251TR-V-AC cells by RNA-FISH assay. Scale bar = 50 μm. b, c Expression of miRNAs in U87TR and U251TR after V-NC or V-AC transfection measured by qRT-PCR. *P < 0.05 compared with U87TR-V-NC or U251TR-V-NC cells. d The luciferase activity of pMIR-lncRNA AC003092.1-Wt and pMIR-lncRNA AC003092.1-Mut after miR-195 or anti-miR-195 transfection in U87TR cells by dual-luciferase reporter assay. *P < 0.05. e The expression of lncRNA AC003092.1 and miR-195 in the complexes using anti-Ago2 antibody by RNA-binding protein immunoprecipitation assay. *P < 0.05 compared with anti-IgG group. Data are presented as mean ± SD of three independent experiments