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. 2018 Oct 11;46(20):10535–10545. doi: 10.1093/nar/gky910

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Published by Oxford University Press on behalf of Nucleic Acids Research 2018.

This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 1. — Strategy to suppress homologous recombination in HIV-1. (A) Control vector pair. The asterisk denotes an inactivating frameshift mutation, whereas ‘I’ indicates an internal ribosome entry site. (B) Vector pair designed to suppress recombination. Recombination was suppressed in 2.5 kb of the viral genome by replacing the wild-type HIV-1 rt and in regions with synthetic HIV-1 rt (blue) or HIV-2 ROD in (red) regions. The effects of suppressing recombination could be determined by analyzing GFP⁺ cells, which could only result from heterozygous particles. (C) Experimental protocol for determining the effects of suppressing recombination on GFP titers and GFP⁺ provirus structures.