. 2018 Oct 11;46(20):10535–10545. doi: 10.1093/nar/gky910

Table 1.

Decreased nucleotide sequence homology blocks homologous recombination

		pro		rt		in		Total
Vector pair	Seq	Cross-overs	Cross-overs/seq^a	Cross-overs	Cross-overs/seq^a	Cross-overs	Cross-overs/seq^a	Cross-overs	Cross-overs/seq^a
H0/T6S	86	28	0.33	113	1.31	40	0.47	181	2.10
H0-SynRT/T6S	130	18	0.14	0	< 0.01^b	56	0.43	74	0.57
H0-SynRT/T6S-IN2	120	14	0.12	0	< 0.01^b	2	0.02	16	0.13

^aThe total number of crossovers within each region was divided by the total number of sequences.

^bNo crossovers were observed between wild-type and synthetic rt for either vector pair, so the rate of crossovers in rt is less than 1/130 or 1/120 (<0.01).