Figure 1.

Growth defects in strains lacking RNase R and DBPs. (A) Growth of strains on LB-agar plates. Deletion alleles of each of the five Escherichia coli DBPs were transferred into WT or Δrnr strain backgrounds. The derivative strains were streaked on an LB-agar plate and grown overnight at 37°C. (B) Dilutions of saturated cultures necessary to yield single colonies were spotted on LB-agar plates and grown overnight at 37°C. (C) Doubling times were determined in LB medium at 37°C. Mean values and standard errors are derived from triplicate cultures for each strain.