Figure 6.

Generation of rRNA fragments due to impaired ribosome assembly. (A) Ribosomal profiles of strains containing mutations in established RAFs. Cell lysates from WT, ΔrimP, ΔrbfA or ΔrimM strains were analyzed by ultracentrifugation, as described for Figure 5. (B and C) RNA was isolated from WT or Δrnr strains containing ΔrimP, ΔrbfA or ΔrimM mutations and analyzed by northern blotting as described in Figure 2 using probes for 16S rRNA (B) or 23S rRNA (C). (D and E) WT or Δrnr strains were grown in the absence or presence of the indicated amounts of antibiotics (Abs) and RNA derived from these strains was analyzed by northern blotting, as described in Figure 2 using probes for 16S rRNA (D) or 23S rRNA (E). (F) Doubling times were determined in LB medium at 37°C. Mean values and standard errors are based on triplicate cultures for each strain.