Figure 6.

The regulatory role of the uncharacterized TF YeiE is involved in maintaining iron homeostasis under iron-limited conditions in E. coli K-12 MG1655. (A) Functional classification of target genes from YeiE genome-wide bindings. The enriched functions are in three groups: amino acid transport/metabolism, carbohydrate transport/metabolism, and inorganic ion transport/metabolism. (B) The proposed regulatory roles of genes down-regulated in the yeiE deletion strain. (C) Growth profile of wild-type and yeiE deletion strain in iron-free M9 minimal medium supplemented with 0, 0.2 mM, 0.3 mM, 0.4 mM of 2,2′-dipyridyl (DPD) (an iron chelator), respectively. The growth curves were determined from at least six independent cultures and significant differences in the stationary phase between wild type and yeiE deletion strain were determined by the Student's t test, P < 0.01.