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. 2018 Oct 10;46(20):10563–10576. doi: 10.1093/nar/gky903

Figure 1.

Figure 1.

Electron micrographs of viroid RNA. (A) PSTVd RNA spread onto water at room temperature showing rod-shaped structures. (B) PSTVd RNA denatured in 3 M urea, 0.1 M Tris–HCl, 0.1 mM ethylenediaminetetraacetic acid, pH 7 and spread onto water at 40 °C; the micrograph shows various structures ranging from rods and tennis rackets to single-stranded circles. (C) PSTVd RNA denatured as in (B), spread onto water at 70 °C showing mostly single-stranded circles. Calibration bars: 100 nm. For details see references (9,11).