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. 2018 Oct 10;46(20):10563–10576. doi: 10.1093/nar/gky903

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 11. — Thermodynamically and kinetically optimal structures of PSTVd. (A) The rod-like native structure of PSTVd is thermodynamically stable up to temperatures of 75 °C in 1 M ionic strength. (B) In the main transition the rod-like structure denatures with high cooperativity in a structure with two hairpins (HPI and II, which denature at even higher temperature (cf. Figure 9). (C) Structures containing HPII and further hairpins are thermodynamically metastable, but kinetically favored during and after synthesis of replication intermediates in ionic strength conditions of plant cells.