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. 2018 Sep 20;46(20):10840–10854. doi: 10.1093/nar/gky848

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — NS5B fidelity characterization using the snG:U_mis assays. (A) The reaction flow chart and products of the snG:U_mis assays. (B and C) Monitoring the snG:U_mis in a time course manner under a series of UTP concentrations for two representative NS5B constructs (WT and AA). The time course data under each UTP concentration were used to calculate the misincorporation rate (rate_mis), and rate_mis values were then fit to the Michaelis–Menten equation to generate the K_M^app and k_mis values. The data points circled by dashed oval were not included in the fitting due to the inhibitory effect observed under the highest two UTP concentrations.