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. 2018 Aug 29;46(20):10905–10916. doi: 10.1093/nar/gky745

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — 2′-O-methyl modification at position 14 of the antisense strand negatively modulates sdRNA efficacy. (A) The frequency of 2′-O-methyl modification per position of the antisense strand in functional (defined as < 24% gene expression remaining) versus non-functional (defined as >44% gene expression remaining) sdRNAs. (B) The efficacy of sdRNA targeting MAP4K4 with and without 2′-O-methyl modification in position 14 of the antisense strand. MAP4K4 expression was analyzed by qPCR in HeLa cells treated with sdRNAs for 72 h (n = 3, mean ± SD; one-way ANOVA P < 0.001).