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. 2018 Nov 15;8:16903. doi: 10.1038/s41598-018-35043-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DNA damage induced by activation of LC PCBs. (A) Jurkat T cells were incubated with increasing concentrations of 3-OHCB 28 and 3′-OHCB 28 and etoposide as positive control. After 48 hrs. of incubation a comet-assay was performed. Olive tail moments are represented in box plots. Differences of the medians in comparison to negative control were investigated using Kruskal-Wallis, followed by a Dunn’s post-hoc test for multiple comparisons (****P < 0.0001). (B) Representative immunofluorescence images of Jurkat T cells treated as described in A. H2Ax-phosphorylation (green, Alexa Fluor 488) was merged with the nuclear counterstain DAPI (blue). (C) Quantitative evaluation of confocal images from Jurkat T cells. At least 58 cells per sample were counted. Left, percentage of cells with <2 or >2 foci/cell. Right, percentage of cells with <5 or >5 foci/cell. (D) Western blot analysis of γH2Ax in Jurkat T cells treated with 3-OHCB 28 and 3′-OHCB 28 and etoposide for 48 hours. Fold differences of H2Ax-phosphorylation were determined by densitometric analysis.