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. 2018 Nov 15;8:16871. doi: 10.1038/s41598-018-35246-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of cell-free culture filtrate of A. oryzae M2040. (A) Experimental design. (B) Time course of the AFB1 accumulation in a mixed liquid of M2040 cell-free culture fermentate and A. flavus mycelial cells. *P < 0.05. **P < 0.01. (C) Conidial numbers and AFB1 production in HT and non HT M2040 fermentate inoculated with different conidial numbers of A. flavus. **P < 0.05. **P < 0.01. (D) Conidial count and AFB1 production in different concentrations of HT and non HT A. oryzae fermentate inoculated with 5 × 105 A. flavus conidia/ml. *P < 0.05. **P < 0.01. ND: Conidia were not detected under a microscope. Fermentate was diluted in sterile distilled water. (E) Conidial count and AFB1 production in different concentrations of HT and non HT M2040 fermentate inoculated with 5 × 10⁵ A. flavus conidia/ml. **P < 0.05. **P < 0.01. Fermentate was diluted in fresh PDB.