Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 15;8:16871. doi: 10.1038/s41598-018-35246-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Comparative genome analyses of A. oryzae M2040 and Afla-Guard. (A) Phylogenetic relationship of A. oryzae and A. flavus isolates. An unrooted phylogeny was generated using the Maximum Likelihood method from 305,543 SNPs across the entire genome. Branch lengths represent the number of substitutions per site. All bootstrap values were ≥94%. Blue and green taxa labels represent A. oryzae and A. flavus, respectively. (B) Deletion profiles in the AF gene cluster. The chromosomal architecture of the AFB1 gene cluster relative to the A. flavus NRRL 3357 genome is shown above the heatmap, where arrows represent genes, and their orientations represents the direction of transcription. The heatmap represents copy number estimates for each non overlapping 100 bp bin across the AF gene cluster. Black and white represent copy numbers of 0 and ≥1, respectively. Bottom bar shows the Afla-Guard heatmap depicting deletions relative to the AF gene cluster containing A. flavus NRRL 3357 EQ963478 scaffold. Windows represent copy number estimates for each non-overlapping 10 kb bin across the scaffold. The chromosomal region containing the AF cluster is outlined with a red box. (C) Genome architecture of examples M2040 lineage specific genes clusters. Microsynteny of regions covering a three gene (top) and 17 gene (bottom) cluster unique to the M2040 genome in comparison to A. oryzae RIB 40, A. flavus NRRL 3357 and Afla-Guard. For each cluster arrows represent genes, and their orientations represents the direction of transcription. Genes colored black are conserved in at least 2 isolates, while genes colored light blue are unique to the M2040 genome. Gray blocks represent genomic regions exhibiting sequence similarity between isolates. Chromosome, or scaffold identifiers containing these loci are listed under each isolate. Gene identifiers are listed for each gene in panel A, and for the range of genes in panel.