Table 2.
Oligonucleotides used in this study.
| Name | Sequence (5′ → 3′) | Purpose |
|---|---|---|
| OMK740 | TCACTGAAAAAGAGCTAAGACTA | 5′ flanking of AFL gpdA |
| OMK741 | TCCCATGACAGTGTCTTCGT | 3′ flanking of AFL gpdA |
| OMK742 | ACCCCAGTACAGTTTCATGCAA | 5′ nest of AFL gpdA |
| OMK743 | TTGCGCAGAAGCCTAGACAAGTC | 3′ nest of AFL gpdA |
| OMK751 | ATGAGTAAAGGAGAAGAACTT | 5′ GFP ORF |
| OMK752 | TTTGTATAGTTCATCCATGC | 3′ GFP ORF |
| OMK754 | AAGTTCTTCTCCTTTACTCATTGTTTAGATGTGTCTGTTG | 3′ AFL gpdA with GFP taila |
| OMK755 | TGGCATGGATGA ACTATACAAAAAGTCATACCTAACAAGTGCT | 5′ AFL gpdA with GFP taila |
| OMK639 | TCGAGAGATGAGGGCTGCCAGCA | 5′ AFL pyrG marker |
| OMK640 | CAGAAGAAAAGGATGATCAATAC | 3′ AFL pyrG marker |
aTail sequence is in italic.