Table 1.

Summary of tRF-5 series with a relative cloning frequency >0.02‰ (reads per mil) in arsenite-treated samples.

Sequence	Origin	Relative sequencing frequency (‰ of total sequencing reads)
		Untreated	Arsenite
GGGGGTATAGCTCAGCGGTAGAGCGCGTGCT	AlaTGC	0.13	0.46
GGGGATGTAGCTCAGTGGTAGAGCGCGCTTC	AlaCGC	1.31	7.01
GGGGGTGTAGCTCAGTGGTAGAGCGCGTGCT	AlaAGC	1.00	5.25
GGCTCTGGCGCAATGGATAGCGCATTGGACT	ArgAGA	0.10	11.66
GGGCCAGTGGCGCAATGGATAACGCGTCTGA	ArgACG	0.08	0.83
GACCGCGTGGCCTAATGGATAAGGCGTCTGA	ArgCGG	0.04	6.86
GGCTCCGTGGCGCAATGGATAGCGCATTGGA	ArgTCT	0.28	13.46
GGTCCCATGGTGTAATGGTTAGCACTCTGGA	GlnCAA	0.02	1.59
GGTTCCATGGTGTAATGGTTAGCACTCTGGA	GlnCAG	0.07	49.50
GGTTCCATGGTGTAATGGTAAGCACTCTGGA	GlnCTG	0.06	2.84
GGCCCCATGGTGTAATGGTTAGCACTCTGGA	GlnTTG	0.01	0.72
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT	GluCTC-S	0.01	52.90
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAATAATTCTCGGCTGC	GluCTC-L	0.00	33.90
GCATGTGGTTCAGTGGTAGAATTCTCGCCTA	GlyGCC	11.31	23.64
GGTAGCGTGGCCGAGCGGTCTAAGGCGCTGG	LeuAAG	1.322	2.95
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC	MetCAT-1	6.494	79.38
AGCAGTGGCGCAGCGGAAGCGTGCTGGGCCC	MetCAT-2	0.13	0.83
GGCTCGTTGGTCTAGGGGTATGATTCTCGCT	ProTGG	2.01	9.01
GGCTTGGTCTAGGGGTATGATTCTCGCTTCA	ProCGG	0.07	8.96
GCTGTGATGGCCGAGTGGTTAAGGCATTGGA	SerCGA	0.03	0.06
GGCCTCGTGGCGCAACGGTAGCGCGTCTGAC	TrpTGG	0.08	1.28
GACCTCGTGGCGCAACGGTAGCGCGTCTGAC	TrpCCA	0.03	4.42
GGCTCCATAGCTCAGGGGTTAGAGCACTGGT	ThrTGT	0.23	1.28

The relative cloning frequency of a tRF was calculated by dividing its read number by the total read number of each experimental group. tRFs were sorted by an alphabetical order of parental tRNA isoforms. For tRNA isoforms sharing the same anticodon, they were sequentially numbered according to the abundance of tRF-5s in the arsenite-treated sample.