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. 2018 Nov 9;9:2557. doi: 10.3389/fimmu.2018.02557

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Copyright © 2018 Myhill, Stolzenbach, Hansen, Skovgaard, Stensvold, Andersen, Nejsum, Mejer, Thamsborg and Williams.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Diet supplementation with inulin alters key immune parameters. Flow cytometric analysis was conducted on peripheral blood mononuclear cells (PBMCs) isolated from heparinised blood samples attained from all animals (n = 31) prior to Trichuris suis infection (day 0), and days 14 and 28 post-infection (p.i.). (A) Representative flow cytometry plots of PBMC-derived CD3⁺ T cells from control-fed and inulin-fed pigs. Numbers represent the percentage of CD3⁺ T cells. (B) Percentage CD3⁺ T cells derived from total PBMC population. (C) Serum antibody levels against T. suis excretory/secretory antigen, expressed as ELISA units (EU). Antibody levels were measured weekly from day 0 to 28 p.i. Data are presented as means and error bars represent SEM (*p ≤ 0.05, ***p ≤ 0.005, by mixed linear model).