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. 2018 Nov 15;8:16852. doi: 10.1038/s41598-018-35341-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schleiferon formation in concurrent cultures of physically separated Serratia plymuthica and Staphylococcus delphini. (a) VOC-collection system: Charcoal purified and sterilized air was split into two air streams of which each entered one culture flask containing either S. plymuthica 4Rx13 or S. delphini 20771. After VOC-enrichment, the two air streams were reunited and funneled over a VOC adsorbent (Porapak) (b) TIC-GC/MS chromatograms of media (blue line), S. delphini mono-culture (pink line), S. plymuthica mono-culture (black line) and S. plymuthica/S. delphini concurrent cultured for 24 hours (red line) (c) EIC-GC/MS chromatograms of m/z 105 representing the base peak of schleiferon A and B (color code for experimental set up see (b). #1: acetoin, #2: 2-phenylethylamine, #3: schleiferon B, #4: schleiferon A; IS = internal standard (N-nonyl acetate, 5 ng); n = 3.