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. 2018 Nov 15;8:16912. doi: 10.1038/s41598-018-35087-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth of glucose uptake mutants with different carbon sources. PAO1 and mutant strains were inoculated at the same OD₆₀₀ in M9-Triton X-100 supplemented with either 0.4% glucose, 0.4% gluconate, 0.1% 2-ketogluconate or 0.5% succinate, as indicated below the bars. Bacterial cell density (OD₆₀₀) was measured after aerate incubation for 24 h at 37 °C. The bars represent average (n = 3) with standard deviation (SD). (a) OM mutants. ΔΔOM, ΔoprB ΔPA2291 mutant. (b). IM mutants. ΔΔIM, ΔgntP ΔkguT mutant. (c). Growth in 0.1% 2-ketogluconate of the indicated mutants. (a,b) Significance was evaluated with One-way Anova and Tukey post-hoc test. (a) According to one-way Anova analysis, growth differences were not statistically significant at 0.05 level. (b,c). *P < 0.5; ****P < 0.0001. All comparisons for which P is not shown were not significant at 0.05 level.