Table 3.
Differential expression of selected P. aeruginosa genes.
| Locusa | Name | Fold Change | Description | ||
|---|---|---|---|---|---|
| PAO1 + vs. PAO1− | GUN + vs. PAO1+ | GUN + vs. PAO1− | |||
| Genes up-regulated in GUN mutant b | |||||
| PA0105 | coxB | 0.8 | 8.2 | 6.5 | cytochrome C oxidase subunit |
| PA1174 | napA | 0.8 | 4.8 | 3.6 | nitrate reductase subunit |
| PA1430 | lasR | 1.0 | 1.7 | 1.8 | transcriptional regulator LasR |
| PA2426 | pvdS | 2.5 | 1.9 | 4.8 | sigma factor PvdS |
| PA2570 | lecA | 0.7 | 4.7 | 3.1 | LecA lectin |
| PA3361 | lecB | 0.6 | 5.2 | 2.9 | fucose-binding lectin PA-IIL |
| PA3477 | rhlR | 0.8 | 2.4 | 1.9 | transcriptional regulator RhlR |
| PA3478 | rhlB | 0.8 | 3.0 | 2.3 | rhamnosyltransferase subunit B |
| PA3479 | rhlA | 0.7 | 3.3 | 2.5 | rhamnosyltransferase subunit A |
| PA3622 | rpoS | 0.9 | 2.6 | 2.4 | sigma factor σS |
| PA3724 | lasB | 0.5 | 7.8 | 3.5 | elastase LasB |
| PA4210 | phzA1 | 0.4 | 6.2 | 2.7 | phenazine biosynthesis protein |
| PA4211 | phzB1 | 0.5 | 7.2 | 3.2 | phenazine biosynthesis protein |
| PA5348 | PA5348 | 0.8 | 3.0 | 2.4 | probable DNA-binding protein |
| Genes down-regulated in the GUN mutant b | |||||
| PA0576 | rpoD | 1.1 | 0.4 | 0.5 | sigma factor σD |
| PA0893 | argR | 0.6 | 0.6 | 0.4 | transcriptional regulator ArgR |
| PA0895 | aruC | 0.5 | 0.3 | 0.2 | acetylornithine aminotransferase |
| PA2259 | ptxS | 2.9 | 0.5 | 1.4 | transcriptional regulator PtxS |
| PA2264 | PA2264 | 3.4 | 0.5 | 1.5 | conserved hypothetical proteinc |
| PA2320 | gntR | 3.7 | 0.3 | 1.1 | GntR transcriptional regulator |
| PA4006 | nadD | 0.9 | 0.5 | 0.5 | NAM adenylyltransferase |
| PA4920 | nadE | 1.5 | 0.5 | 0.8 | NAD synthetase |
| PA5100 | hutU | 0.8 | 0.0 | 0.0 | urocanate hydratase |
| PA5105 | hutC | 0.8 | 0.2 | 0.1 | Transcriptional regulator HutC |
| Genes for glucose IM transporters deleted in this work | |||||
| PA2262 | kguT | 1.3 | 0.3 | 0.4 | 2-ketogluconate transporter |
| PA2322 | gntP | 35.5 | 0.0 | 0.6 | gluconate permease |
| PA3187 | gltK | 52.0 | 0.1 | 6.7 | ABC transporter ATP-binding protein |
| PA3188 | gltG | 31.6 | 0.0 | 0.4 | sugar ABC transporter permease |
| PA3189 | gltF | 3.5 | 0.3 | 0.9 | probable permease of ABC sugar transporter |
aBoldface characters, genes analysed by RT-qPCR.
bGenes up- and down-regulated in GUN mutant with respect to PAO1 (with and/or without glucose).
cThe protein contains a domain belonging to the Gluconate 2-dehydrogenase subunit 3 family (InterPro IPR027056).