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. 2018 Nov 9;6:159. doi: 10.3389/fbioe.2018.00159

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Copyright © 2018 Mindt, Walter, Risse and Wendisch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic overview of the reaction catalyzed by γ-glutamylmethylamide synthetase (GMAS) and N-methylglutamate synthase (NMGS) and their incorporation in the metabolism of P. putida KT2440. N-methylglutamate formation (thick arrows) takes place by heterologous expression of gmaS and mgsABC from M. extorquens DM4 (green boxes) starting from l-glutamate. Deletion of the transcriptional repressor gene of the glycerol metabolism (glpR) is indicated by red cross. The second copy of endogenous glutamate dehydrogenase (gdhA) under control of Ptac was integrated in glpR locus (blue boxes). Reactions are indicated by an arrow, multiple reactions by dashed arrows.