Table 1.
Strains and vectors used in this work.
| Strains and vectors | Description | Source |
|---|---|---|
| STRAINS | ||
| P. putida KT2440 | P. putida mt-2 hsdRl hsdM | Bagdasarian et al., 1981; Franklin et al., 1981 |
| P. putida KT2440 Δupp | P. putida mt-2 hsdRl hsdM Δupp | Graf and Altenbuchner, 2011 |
| P. putida KT2440 Δupp ΔglpR::Ptac-gdhA | P. putida mt-2 hsdRl hsdM Δupp ΔglpR::Ptac-gdhA | This work |
| E. coli DH5α | F-thi-1 endA1 hsdr17(r-, m-) supE44 ΔlacU169 (Φ80lacZΔM15) recA1 gyrA96 | Hanahan, 1983 |
| M. extorquens DM4 | Wild type DM4 | Gälli and Leisinger, 1985; Vuilleumier et al., 2009 |
| NMG0 | P. putida KT2440 carrying pEV1 | This work |
| NMG1 | P. putida KT2440 carrying pEV1-mgsABC | This work |
| NMG2 | P. putida KT2440 carrying pEV1-mgsABC-gmaS | This work |
| NMG3 | P. putida KT2440 Δupp ΔglpR::Ptac-gdhA carrying pEV1-mgsABC-gmaS | This work |
| VECTORS | ||
| pEV1 | Expression shuttle vector pACYC184 derivative containing TetR, pVS1_ori, p15A_ori,rhaPBAD expression system | Provided by Evonik Creavis GmbH |
| pEV1-mgsABC-gmaS | pEV1 derivative for l-rhamnose inducible expression of mgsABC-gmaS from M. extorquens DM4 containing an artificial RBS 5′-CTAGGAGGATTCGTC-3′ | This work |
| pEV1-mgsABC | pEV1 derivative for l-rhamnose inducible expression of mgsABC from M. extorquens DM4 containing artificial RBS 5′-CTAGGAGGATTCGTC-3′ | This work |
| pKOPp | pJOE6261.2 derivative for deletions in P. putida KT2440 | Graf and Altenbuchner, 2011 |
| pKOPp-glpR::Ptac-gdhA | pJOE6261.2 derivative for deletion of glpR in P. putida KT2440 and integration of gdhA containing an artificial RBS 5′-CTAGGAGGATTCGTC-3′ under the control of the constitutive promotor Ptac | This work |