Table 3.

Protein-protein sequence comparison.

Used protein sequence*#	Blastp-best hit	Identity (%)	Conserved protein domain family
GMAS (gmaS; METDI2327)	type III glutamate-ammonia ligase Bosea vaviloviae (WP_069689945.1, [432 aa])	78	Family: glutamine synthetase, type III
NMGS I (mgsA; METDI2324)	glutamine amidotransferase Rhizobiaceae bacterium (WP_114956843.1, [300 aa])	72	Glutamine amidotransferases class-II (Gn-AT)_GlxB-type; Glutamate synthase domain 1 (GltB1)
NMGS II (mgsB; METDI2325)	protein glxC Ancylobacter rudongensis (WP_091437349.1, [225 aa])	80	Glutamate synthase domain 3 (GltB3)
NMGS III (mgsC; METDI2326)	FMN-binding glutamate synthase family protein Novosphingobium acidiphilum (WP_028640033.1, [442 aa])	88	Glutamate synthase (GltS) FMN-binding domain; Glutamate synthase domain 2 (GltB2)

Protein sequences of GMAS, NMGS (I, II, III) of M. extorquens DM4 were analyzed within the bacterial taxid:2 using the algorithm blastp (Altschul et al., 1990). The best hits for each protein BLAST (lowest e-value) are shown. The predicted protein function, conserved protein domain family and the sequence identity are shown.

^*Amino acid sequence analysis was performed with blastp (https://blast.ncbi.nlm.nih.gov/Blast.cgi).

^#Search within the bacterial taxid:2, excluding the genus Methylobacterium.