Table 3.
Pass rates for qPCR runs and samples (DNA input).
| Total (n) | Valid results | Invalid results | Rate [%] | |
|---|---|---|---|---|
| Run pass rate - qPCR run performed | 6 | 6 | 0 | 100 |
| Sample pass rate - first-time | 104 | 91 | 13 | 87.5 |
| Sample pass rate - re-test | 104 | 99 | 5 | 95.2 |
All criteria for run validity and sample DNA input validity were automatically applied according to the PITX2 Assay Profile of the therascreen PITX2 RGQ PCR Kit18. All determinations were performed in duplicates.