Fig. 1. Endogenous PLOD3 plays an important role in lung cancer.
a Representative microscopic images of lung cancers and their normal tissue counterparts stained with an anti-PLOD3 antibody (left panel, scale bar, 100 μm). Staining intensity was scored as follows in all patient samples (n = 59). The scores are calculated by multiplying the staining intensity with the percentage of stained cells in primary lung cancer relative to the paired normal tissue. Statistical significance was determined by Student’s t-test. ***P < 0.001. b PLOD3 upregulation is positively correlated with the stage of lung cancer in all patient samples (n = 59). c Box plot analysis of the PLOD3 mRNA levels obtained from ONCOMINE from lung cancer patients. Gene: PLOD3; Analysis type: cancer vs normal analysis; Data type: mRNA; Sample type: clinical specimen; Lung. d The effects of PLOD3 on the overall survival of lung cancer patients, using Kaplan–Meier plotter analysis. e R-H460 and A549 cells were cultured up to 80% confluence, followed by changing of the medium to fresh serum-free RPMI1640. After 6 h, collected supernatants were analyzed via immunoblotting with anti-PLOD3 antibody (left panel) and enzyme-linked immunosorbent assay (ELISA) at 450 nm (right panel). Statistical significance was determined by Student’s t-test. ***P < 0.001. f R-H460 and A549 cells (1 × 106 cells) were inoculated subcutaneously into the backs of nude mice. After tumorigenesis, mice were killed. Serum collected from each group was analyzed via ELISA. Statistical significance was determined by Student’s t-test. **P < 0.01