Fig. 3. Loss of PLOD3 suppressed R-H460 tumor metastasis in vivo.

a Representative micrographs with metastatic nodules are shown via Bouin’s staining. b Hematoxylin and eosin staining were performed using xenografts. c, d The number and volume of metastatic nodules were determined microscopically in PLOD3 xenograft tumors. Statistical significance was determined by Student’s t-test. *P < 0.05, **P < 0.01. e, f Estimation of hPLOD3 mRNA and PLOD3 protein levels in the lung nodules. Statistical significance was determined by ANOVA analysis. ***P < 0.001