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. 2018 Nov 15;8:16853. doi: 10.1038/s41598-018-35203-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Results of significant canonical pathways associated with ion-transport and the molecules involved. (a) Calcium Transport- I pathway. (b) cAMP-mediated signaling pathway. (c) Cardiac-β-adrenergic signaling pathway. The canonical pathways were analyzed using QIAGEN’s Ingenuity Pathway Analysis (IPA; QIAGEN Inc, https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis)³². Differentially expressed genes in the layers were subjected to IPA analysis and significant canonical pathways were identified at P-value < 0.05. Above identified canonical pathways demonstrate how the candidate molecules (genes) are involved in ion-transport.