Table 2.
Function | Primer | Sequence |
---|---|---|
Cloning of caeA into pNZ44 | CaeAF | gctcga ccatgg atcagcgt tcatcattcg∗ |
Cloning of caeA into pNZ44 | CaeAR | ctctgc tctaga gaatgtccgc gcagccgtac |
Cloning of caeA with His tag into pNZ8150 | CaeAHisF | cctgca gatatc atgcatcaccat caccatcacca tcaccatcacgacat caaaccgtgggaatac |
Cloning of caeA with His tag into pNZ8150 | CaeAHisR | ctctgc tctaga gaatgtccgc gcagcc gtac |
∗Restriction enzyme sites are highlighted in bold.