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. 2018 Nov 15;8:16833. doi: 10.1038/s41598-018-34968-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time dependence of the voltage present between the electrodes under the unipolar and bipolar settings. (A) Electric parameters and corresponding voltage profiles. A 1 s delay was present between each HV pulse. This was obtained by the settings of the B10 electropulsator keeping the 1 Hz frequency for the delivery of trains. (B) tdTomato expression was followed over time by non-invasive fluorescence microscopy. Values are means ± s.e.m., n = 4. ns = non-significant result (Two-way ANOVA analysis).