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. 2018 Nov 15;8:16833. doi: 10.1038/s41598-018-34968-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels for dtTomato for male and female mice. 25 µg of plasmid DNA coding the fluorescent protein tdTomato were intradermally injected in the back of anesthetized C57Bl/6 mice (female ○, male Δ) and the electrotransfer was performed using the HV-MV (100 µs, 400 V + 20 ms, 100 V) parameters. tdTomato expression was followed over time by non-invasive fluorescence microscopy. Values are means ± s.e.m., n = 4. **P < 0.01 (Two-way ANOVA analysis).