Figure 8.

pIL-12 GET induces expression and secretion of IL-12. Mice were intradermally injected with 25 µg of pIL-12 in 20 µl PBS, or with 20 µl of PBS alone in one flank and were submitted to HV-MV electrical treatment. The contralateral flank from pIL-12 injected mice was non-injected and non-pulsed to be used as an internal control. Mice were sacrificed from 1 to 14 days after treatment and skins from each flank were harvested and cultured overnight in complete medium 5% CO₂, 37 °C. IL-12 content was determined by ELISA in the cultured supernatant of pIL-12 treated skin (IL-12 + GET), of PBS treated skin (PBS + GET) and in contralateral untreated skin from pIL-12 GET treated mice (IL-12). (n = 5, 3 independent experiments). Statistical analysis: 2way Anova, *p < 0.05, **p < 0.005.