Fig. 3. Inhibition of JNK using SP600125 reduces necrosome formation in macrophages.

(a–c) Peritoneal macrophages were pretreated with zVAD, DMSO, or SP600125 for 30 min, followed by TNF (a), poly I:C (b), or LPS (c) treatment for the indicated times. Lysates were analyzed by immunoblotting with the indicated antibodies. d, e Immunoblot analysis with indicated antibodies of RIPK1 or mouse IgG immunoprecipitates and total lysates from peritoneal macrophages treated with TNF+zVAD (d) and poly I:C+zVAD (e) for indicated periods of time. f, g Peritoneal macrophages were treated by TNF (f) or poly I:C (g) as in d or e. Lysates were analyzed by immunoblotting with antibodies against pMLKL, RIPK3, or GAPDH. Data are representative of at least three independent experiments