Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 15;8:16904. doi: 10.1038/s41598-018-35161-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fungi-specific T cell proliferation. (A–C) Purified CD4⁺CD45RA⁺ T cells were labelled with CFSE and cultured with monocytes matured with heat-inactivated C. albicans or A. fumigatus at a ratio of 2:1. CFSE dilution profiles and the frequency of proliferating (CFSE^lo) T cells from neonates (A), infants and children (C) or adults (B) on day 3 and day 6 after stimulation. Data are representative of 5 donors. (D) Frequency of proliferating (CFSE^lo) T cells from neonates, infants, children, and adults stimulated with C. albicans (orange), A. fumigatus (blue) or anti-CD3/CD28 (black) determined by flow cytometry are plotted against age. The dotted lines represent the 95% confidence interval. The coefficient of determination (R²) according to the one-phase decay exponential model in response to C. albicans-antigen is 0,9209. (E) Bar graphs showing frequency of naïve (CD4⁺CD45RA⁺CD31⁺) or memory (CD4⁺CD45RO⁺) T cells of children, expressing CD25 upon stimulation with Tetanus Toxoid for 3 days. Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. ****p < 0.0001, as determined by one-way Anova with Tukey post hoc test.