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. 2018 Nov 15;8:16905. doi: 10.1038/s41598-018-34897-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bursal B cells rapidly increased at the late embryonic stage in chickens. Bursal cells were taken from chicks aged E13 to 2–6 days old and stained with anti-Bu-1 antibody, which were regarded as bursal B cells. (a) Changes in bursal B cell population at E15, E17, E18, and hatching (H). Numbers in each area indicate the percentage of non-B or B cells in the bursa. (b) The percentage and absolute number of bursal B cells during embryonic and post-hatching stages. Each data point represents the mean value of four to ten chickens. The frequency of B cells in comparison to non-B cells from the spleen (c) and bone marrow (d) at the indicated stage. More than 5 samples per embryonic stage were analyzed, and all results represent at least two independent experiments. Each data point represents the mean of four to ten chickens with SD. **P < 0.01, ***P < 0.001.