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. 2018 Nov 15;8:16845. doi: 10.1038/s41598-018-35430-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proximity Ligation Assay on Scramble/Cas9 HeLa cells showing interactions between TMEM97 and PGRMC1, LDLR and PGRMC1, and LDLR and TMEM97. (A) standard, non-lipoprotein depleted conditions, (B) sterol-starved conditions, and (C) sterol-starved conditions followed with treatment with 50 μg/mL LDL for 3 hours. Inserts represent an enlarged region focusing on a single cell.