Table 2. Effect of hydrolytic enzymes (final concentration of 1 mg/mL) on antimicrobial activity of the CFS obtained from E. faecalis KT11.
| Treatments | Inhibition zone diameter (mm)a | ||
|---|---|---|---|
| S. aureus ATCC 25923 | P. aeruginosa ATCC 2783 | M. luteus NRRL 1018 | |
| CFS (control) | 15.0±0.5 | 18.0±1.0 | 15.0±0.8 |
| Trypsin+CFS | -b | - | - |
| Trypsin+buffer | - | - | - |
| Pepsin+CFS | 9.0±1.4 | 10.0±2.9 | 10.0±1.8 |
| Pepsin+buffer | - | - | - |
| α-Chymotrypsin+CFS | - | - | - |
| α-Chymotrypsin+buffer | - | - | - |
| Protease+CFS | - | - | - |
| Protease+buffer | - | - | - |
| Proteinase K+CFS | - | - | - |
| Proteinase K+buffer | - | - | - |
| Catalase+CFS | 15.0±0.8 | 16.0±0.5 | 16.0±0.5 |
| Catalase+buffer | - | - | - |
| α-Amylase+CFS | 15.0±1.0 | 16.0±1.6 | 16.0±1.0 |
| α-Amylase+buffer | - | - | - |
a Agar well assay was used and wells (8 mm in diameter) were filled with 100 µL CFS samples
b no inhibition.