TABLE 2.

Primers and probes used for the study

Namea	Sequenceb
ddPCR
Bl-04_F	5′-CTT CCC AGA AGG CCG GGT-3′
Bl-04_P	5′-6-FAM/CGA AGA TGA/ZEN/TGT CGG AAC ACA AAC ACC CGG/3IABkFQ-3′
Bl-04_R	5′-CGA GGC CAC GGT GCT CAT ATA GA-3′
HN019_F	5′-TTC GAT GGT TCG CAC AGT GA-3′
HN019_P	5-6-FAM-AAA CAG GTC/ZEN/AAT CAG CGG CGC AGG GAG/3IABkFQ-3′
HN019_R	5′-GGT CTG ATG CCG CCT GAA AT-3′
SNPs
Indel_2_F	GCC GCA GAT CGA ATA CTG GG
Indel_2_R	AAG CGA CGA CCG AAT GCA AT
Indel_4_F	TCT GTT GCG GGA TGT CAT GC
Indel_4_R	GGC GAT TCA GGC GAA GTT CA
Indel_7_F	ACA GAA GTA GGC GAG GGG AC
Indel_7_R	AAC ATC ACC GCC GAT GAA CC
Indel_9_F	GGA ACT TGG CAG ACG TCT CG
Indel_9_R	CTG TTG ACT CCG GCT GCA TT
Indel_11_F	GCA GAT CGC CCC ATT GAA CA
Indel_11_R	TCG GGT CTG CTC GAC ATT CT
Indel_14A_F	CAC ACG ATC GGA ACC AGT GG
Indel_14A_R	ACG AAC GAA GTA GCC GAG GA
Indel_14B_F	CGC CGA ATC GCC ATA ATC CA
Indel_14B_R	CAG CCC GAA TCC ACT TGA CC
Indel_17_F	CTC AGT GTG CAC GCA CTC C
Indel_17_R	GAT GCA GGT TGA GCA AGG CG
Indel_19_F	CCT CGC TGT TTC GCT CTG AG
Indel_19_R	GGA AGG TGA CAT GCA GAC CG
Indel_20_F	AAC GAT CAT TTC CGC CAC CC
Indel_20_R	ATG CTG TTC GAT GCG TTG GT
Indel_22_F	CAT CCA CAG CAG CCA ACT CA
Indel_22_R	CCT GAA CCA GAT TGC CAC CG
Indel_23_F	TTC GTC ACT GGA TCG CAA GC
Indel_23_R	TCC AAC AAA CTC ACC GTG GC
Indel_27_F	GCT CTT CGT CTT CGC GGT AC
Indel_27_R	CCG ACA ATC TGC GGC AAT GA

^addPCR, droplet digital PCR; SNP, single-nucleotide polymorphism.

^bZEN, ZEN quencher (IDT); IABkFQ, Iowa Black fluorescence quencher.