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Primers used for MiSeq amplification of the ITS1 gene in fungi
| Primer by PCR round | Sequence (5′ to 3′)a |
|---|---|
| First-round PCR | |
| ITS1Fngs | ACACTCTTTCCCTACACGACGCTCTTCCGATCTGGTCATTTAGAGGAAGTAA |
| ITS2 | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGCTGCGTTCTTATCGATGC |
| Second-round PCR | |
| Generic forward | AATGATACGGCGACCACCGAGATCTACAC[index1]ACACTCTTTCCCTACACGAC |
| Generic reverse | CAAGCAGAAGACGGCATACGAGAT[index2]GTGACYGGAGTTCAGACGTGT |
The two indices (index1 and index2) represent base indices (Illumina).
