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Primers used for MiSeq amplification of the 16S rRNA gene in bacteria
| Primer by PCR round | Sequence (5′ to 3′)a |
|---|---|
| First-round PCR | |
| Forward | ACACTCTTTCCCTACACGACGCTCTTCCGATCTACGCGHNRACCTTACC |
| Reverse | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTACGGGCRGTGWGTRCA |
| Second-round PCR | |
| Forward | AATGATACGGCGACCACCGATCTACA[index1]ACACTCTTTCCCTACACGAC |
| Reverse | CAAGCAGAAGACGGCATACGAGAT[index2]GTGACTGGAGTTCAGACGTGT |
The two indices (index1 and index2) represent base indices (Illumina).
