TABLE 3.

List of mutations in genes encoding glycan biosynthesis pathways detected in BIMs generated in this study

BIMa	Mutated gene	Putative gene function	Type of mutation	AAb position	Phenotypic change compared to WT
STCH_09_BIM	epsH	Glycosyltransferase	AA substitution	262	Reduced adsorption, enhanced viscosity
	rgp	Glycosyltransferase	Gene truncation	197
STCH_13_BIM	epsD	Tyrosine-kinase	AA insertion	160	Not detected
STCH_14_BIM	epsE	Priming glycosyltransferase	Gene truncation	37	Long chains
STCH_15_BIM_1	epsE	Priming glycosyltransferase	Gene deletion	Whole gene	Reduced adsorption, long chains
STCH_15_BIM_2	epsK	Glycosyltransferase	AA substitution	75	Reduced adsorption, long chains
STCH_15_BIM_3			Gene truncation	93	Reduced adsorption, long chains
STCH_15_BIM_4			Gene truncation	82	Reduced adsorption, long chains
STCH_15_BIM_5			Gene truncation	93	Reduced adsorption, long chains

^aBIM, bacteriophage-insensitive mutant.

^bAA, amino acid.