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. 2018 Nov 15;84(23):e02068-18. doi: 10.1128/AEM.02068-18

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FIG 1 — Western blot analysis of Sph2 production from three strains of L. interrogans serovar Pomona. Western blots from three L. interrogans Pomona strains grown in EMJH medium were probed with Sph2 and LipL41 antisera. The Sph2 antiserum cross-reacts with SphH. L. interrogans serovar Manilae strain L495 was grown in EMJH medium supplemented with 120 mM sodium chloride, and its lysate was included for the comparison of Sph2 levels. Sph2 produced by the L495 strain has a slightly lower molecular weight than Sph2 produced by Pomona strains.